Reba Howard

Rebecca J Howard, PhD

Biochemist

Publications

Biophysical characterization of the SARS-CoV-2 E protein

bioRxiv 2021.05.28.446179, 2021 (preprint)

Mehregan A, Pérez-Conesa S, Zhuang Y, Elbahnsi A, Pasini D, Lindahl E, Howard RJ, Ulens C, Delemotte L

Probing solution structure of the pentameric ligand-gated ion channel GLIC by small-angle neutron scattering

bioRxiv 2021.04.10.439285, 2021 (preprint)

Lycksell M, Rovšnik U, Bergh C, Johansen NT, Martel A, Porcar L, Arleth L, Howard RJ, Lindahl E

A cationic lipid site at the outward transmembrane face of a pentameric ligand-gated ion channel

bioRxiv 2021.03.24.436810, 2021 (preprint)

Sridhar A*, Lummis SCR*, Pasini D, Mehregan A, Brams M, Kambara K, Bertrand D, Lindahl E, Howard RJ, Ulens C

Markov state models of proton- and gate-dependent activation in a pentameric ligand-gated ion channel

bioRxiv 2021.03.12.435097, 2021 (preprint)

Bergh C, Heusser SA, Howard RJ, Lindahl E

Dynamic closed states of a ligand-gated ion channel captured by cryo-EM and simulations

bioRxiv 2021.01.04.425171, 2021 (preprint)

Rovšnik U, Zhuang Y, Forsberg BO, Carroni M, Yvonnesdotter L, Howard RJ, Lindahl E

Direct detection of SARS-CoV-2 using non-commercial RT-LAMP reagents on heat-inactivated samples

Scientific Reports 11: 1820, 2021

Alekseenko A, Barrett D, Pareja-Sanchez Y, Howard RJ, Strandback E, Ampah-Korsah H, Rovšnik U, Zuniga-Veliz S, Klenov A, Malloo J, Ye S, Liu X, Reinius B, Elsässer S, Nyman T, Sandh G, Yin X, Pelechano V

Arrangement and symmetry of the fungal E3BP-containing core of the pyruvate dehydrogenase complex

Nature Communications 11: 4667, 2020

Forsberg BO, Aibara S, Howard RJ, Mortezaei N, Lindahl E

Shared structural mechanisms of general anaesthetics and benzodiazepines

Nature 585: 303 – 308, 2020

Kim JJ, Gharpure A, Teng J, Zhuang Y, Howard RJ, Zhu S, Noviello CM, Walsh RM, Lindahl E, Hibbs RE

Agonist selectivity and ion permeation in the α3β4 ganglionic nicotinic receptor

Neuron 104(3): 501 – 511.E6, 2019

Gharpure A, Teng J, Zhuang Y, Noviello CM, Walsh RM, Cabuco R, Howard RJ, Zaveri NT, Lindahl E, Hibbs RE

Sharing data from molecular simulations

Journal of Chemical Information and Modeling 59(10): 4093 – 4099, 2019

Abraham M, Apostolov R, Barnoud J, Bauer P, Blau C, Bonvin AMJJ, Chavent M, Chodera J, Čondić-Jurkić K, Delemotte L, Grubmüller H, Howard RJ, Jordan EJ, Lindahl E, Ollila OHS, Selent J, Smith DGA, Stansfeld PJ, Tiemann JKS, Trellet M, Woods C, Zhmurov A

Structural basis for a bimodal allosteric mechanism of general anesthetic modulation in pentameric ligand-gated ion channels

Cell Reports 23(4): 993 - 1004, 2018

Fourati Z*, Howard RJ*, Heusser SA, Hu H, Ruza RR, Sauguet L, Lindahl E, Delarue M

*equal contributions

Functional characterization of neurotransmitter activation and modulation in a nematode model ligand-gated ion channel

Journal of Neurochemistry 138(2): 243 – 253, 2016

Heusser SA, Yoluk Ö, Klement G, Riederer EA, Lindahl E, Howard RJ

Functional validation of virtual screening for novel agents with general anesthetic action at ligand-gated ion channels

Molecular Pharmacology 84(5): 670 – 678, 2013

Heusser SA, Howard RJ, Borghese CM, Cullins MA, Brömstrup T, Lee US, Lindahl E, Carlsson J, Harris RA

Inhibition versus potentiation of ligand-gated ion channels can be altered by a single mutation that moves ligands between intra- and intersubunit sites

Structure 21(8): 1307 - 1316, 2013

Brömstrup T, Howard RJ, Trudell JR, Harris RA, Lindahl E

Structural basis for potentiation by alcohols and anaesthetics in a ligand-gated ion channel

Nature Communications 4: 1697, 2013

Sauguet L*, Howard RJ*, Malherbe L, Lee US, Corringer P-J, Harris RA, Delarue M

*equal contributions

Multisite binding of a general anesthetic to the prokaryotic pentameric Erwinia crysanthemi ligand-gated ion channel (ELIC)

Journal of Biological Chemistry 288(12): 8355 – 8364, 2013

Spurny R, Billen B, Howard RJ, Brams M, Debaveye S, Price K, Weston D, Strelkov S, Tytgat J, Bertrand S, Bertrand D, Lummis S, Ulens C

Structural insight into KCNQ (Kv7) channel assembly and channelopathy

Neuron 53(5): 663 – 675, 2007

Howard RJ, Clark KA, Holton JM, Minor DL

The structure of the APPBP1-UBA3-NEDD8-ATP complex reveals the basis for selective ubiquitin-like protein activation by an E1

Molecular Cell 12(6): 1427 – 1437, 2003

Walden H, Podgorski MS, Huang DT, Miller DW, Howard RJ, Minor DL, Holton JM, Schulman BA

Ring-closing metathesis of olefinic peptides: design, synthesis, and structural characterization of macrocyclic helical peptides

Journal of Organic Chemistry 66(16): 5291 – 5302, 2001

Blackwell HE, Sadowsky JD, Howard RJ, Sampson JN, Chao JA, Steinmetz WE, O’Leary DJ, Grubbs RH

Selected Talks

Transmembrane mechanisms of allosteric modulation in pentameric ion channels: from bacteria to brain

Austin, TX 2020 (remote)

UT-Austin Ion Channel Biophysics

Allosteric modulation via an intersubunit transmembrane site in Cys-loop receptors

Kyoto, Japan 2018

International Society for Biomedical Research on Alcoholism

Structural details of an allosteric mechanism for bimodal anesthetic modulation of pentameric ligand-gated ion channels

San Francisco, CA 2018

Biophysical Society

Alcohol at the interface: Functional and computational insights from prokaryotic Cys-loop receptors

New Orleans, LA 2016

Research Society on Alcoholism

Alcohol binding to ligand-gated ion channels revealed in a prokaryotic model system

Portland, ME 2013

University of New England

Alcohol modulation of ligand-gated ion channels: Insights and opportunities from new structures

Stockholm, Sweden 2013

Science for Life Laboratory

Simple structural models for alcohol modulation of ligand-gated ion channels

Albany, NY 2012

Albany Medical College

A combined functional-computational approach to characterizing structural sites of anesthetic modulation

San Diego, CA 2012

American Chemical Society

Structural basis for alcohol modulation of pentameric ligand-gated ion channels

San Francisco, CA 2012

Biophysical Society

Structural basis for alcohol modulation of a pentameric ligand-gated ion channel

Stockholm, Sweden 2011

Stockholm University

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